글번호
5033349
일 자
16.03.04
조회수
146
글쓴이
김은아
제목 : 2016-03-04 저널 Dynamic DNA binding licenses a repair factor to bypass roadblocks in search of DNA lesions. (김병주)
2016 Feb 3;7:10607. doi: 10.1038/ncomms10607.

Dynamic DNA binding licenses a repair factor to bypass roadblocks in search of DNA lesions.

Abstract

DNA-binding proteins search for specific targets via facilitated diffusion along a crowded genome. However, little is known about how crowded DNA modulates facilitated diffusion and target recognition. Here we use DNA curtains and single-molecule fluorescence imaging to investigate how Msh2-Msh3, a eukaryotic mismatch repair complex, navigates on crowded DNA. Msh2-Msh3 hops over nucleosomes and other protein roadblocks, but maintains sufficient contact with DNA to recognize a single lesion. In contrast, Msh2-Msh6 slides without hopping and is largely blocked by protein roadblocks. Remarkably, the Msh3-specific mispair-binding domain (MBD) licences a chimeric Msh2-Msh6(3MBD) to bypass nucleosomes. Our studies contrast how Msh2-Msh3 and Msh2-Msh6 navigate on a crowded genome and suggest how Msh2-Msh3 locates DNA lesions outside of replication-coupled repair. These results also provide insights into how DNA repair factors search for DNA lesions in the context of chromatin.

첨부파일 첨부파일:
첨부파일이 없습니다.
목록으로
다음글 2016-03-18 저널 Comparative genetics. Systematic discovery of cap-independent translation sequences in human and viral genomes. (유진배)
이전글 2016-02-26 저널 5' UTR m(6)A Promotes Cap-Independent Translation.(홍가영)