글번호
5705524
일 자
16.08.25
조회수
59
글쓴이
김은아
제목 : 2016-08-05 Tunable Single-Cell Extraction for Molecular Analyses. (김병주)
2016 Jul 14;166(2):506-16. doi: 10.1016/j.cell.2016.06.025.

Tunable Single-Cell Extraction for Molecular Analyses.

Abstract

Because of cellular heterogeneity, the analysis of endogenous molecules from single cells is of significant interest and has major implications. While micromanipulation or cell sorting followed by cell lysis is already used for subsequent molecular examinations, approaches to directly extract the content of living cells remain a challenging but promising alternative to achieving non-destructive sampling and cell-context preservation. Here, we demonstrate the quantitative extraction from single cells with spatiotemporal control using fluidic force microscopy. We further present a comprehensive analysis of the soluble molecules withdrawn from the cytoplasm or the nucleus, including the detection of enzyme activities and transcript abundances. This approach has uncovered the ability of cells to withstand extraction of up to several picoliters and opens opportunities to study cellular dynamics and cell-cell communication under physiological conditions at the single-cell level.

Copyright © 2016 Elsevier Inc. All rights reserved.

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