글번호
5469684
일 자
16.06.17
조회수
151
글쓴이
김은아
제목 : 2016년 6월 17일 Regulation of Poly(A) Tail and Translation during the Somatic Cell Cycle. (구소현)
2016 May 5;62(3):462-71. doi: 10.1016/j.molcel.2016.04.007.

Regulation of Poly(A) Tail and Translation during the Somatic Cell Cycle.


Abstract

Poly(A) tails are critical for mRNA stability and translation. However, recent studies have challenged this view, showing that poly(A) tail length and translation efficiency are decoupled in non-embryonic cells. Using TAIL-seq and ribosome profiling, we investigate poly(A) tail dynamics and translational control in the somatic cell cycle. We find dramatic changes in poly(A) tail lengths of cell-cycle regulatory genes like CDK1, TOP2A, and FBXO5, explaining their translational repression in M phase. We also find that poly(A) tail length is coupled to translation when the poly(A) tail is <20 nucleotides. However, as most genes have >20 nucleotide poly(A) tails, their translation is regulated mainly via poly(A) tail length-independent mechanisms during the cell cycle. Specifically, we find that terminal oligopyrimidine (TOP) tract-containing transcripts escape global translational suppression in M phase and are actively translated. Our quantitative and comprehensive data provide a revised view of translational control in the somatic cell cycle.

Copyright © 2016 Elsevier Inc. All rights reserved.

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